In vitro Evaluation of Antibiofilm Activity of Methanolic Leaf Extract of Azadirachta indica on Cariogenic Streptococcus mutans

Dental caries is a biofilm-dependent disease, and Streptococcus mutans the primary etiological agent involved in initiation of disease. The extensive use limited range antimicrobial drugs dentistry has led to development drug-resistant bacteria. There an increasing need find new alternatives against Globally, there continuous effort towards identifying natural anti-caries agents for prevention better management caries. objective present study was evaluate antibiofilm potential Azadirachta indica leaf methanolic (ALM) extract S. biofilm. employed standard reference strain MTCC 497, vitro standardisation biofilm by microtiter plate assay. activity ALM evaluated strain, same confirmed light scanning electron microscopy (SEM). results demonstrated that 50 µl/ml inoculum concentration exhibited much superior formation than other concentrations employed. Light SEM images revealed at 100 mg/ml significantly inhibited To conclude, reports A. source inhibit Further studies are warranted identify phytochemicals responsible aid design products.